Université Versailles-Saint-Quentin-en-Yvelines
Enseignement
La personne recrutée effectuera des enseignements en licence à l’UFR Sciences (campus de Versailles). Les enseignements concernent principalement des UE de génétique et de biologie cellulaire et moléculaire.
Recherche
- La personne recrutée effectuera sa recherche au sein du Laboratoire de Génétique et Biologie Cellulaire situé à l’UFR Santé-Simone Veil (Montigny-Le-Bretonneux).
- Projet de recherche : ROS and intestinal homeostasis
The project aims to examine the localization and impact of reactive oxygen species (ROS), more specifically H2O2 in intestinal homeostasis. ROS are necessary for defense against pathogens, but too much ROS can have harmful effects. In intestinal pathophysiology, ROS are involved in chronic inflammatory diseases.The Drosophila model will be used for its ease of obtaining mutants and since the processes involved in intestinal regeneration are conserved with mammals. The project will address the following questions: in physiological or pathophysiological conditions can we detect an H2O2 modulation in enterocytes ? Where inside the cell ? How do the different sources of ROS contribute to the H2O2 signal? What are the relationship between the ROS sources? What are the consequences of modulating H2O2 production at the physiological and molecular levels? Is H2O2 a signaling messager between cells in the gut? To answer these questions, the post-doctoral fellow will make use of a genetically encoded fluorescent probe for H2O2. The biosensor can be addressed to different cells and at different locations inside the cell. The depletion or inhibition of specific ROS producing enzymes or complexes on this H2O2 signal will be examined. Then, H2O2 modulation impact will be assessed at the physiological level, especially with different proliferation assays in the gut, and at the molecular level.
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