Eukaryotic cells contain extensive internal membranes defining many compartments having each specific functions. However these organelles are constantly moving and reshaping, still retaining their identity. The maintenance of cell compartmentalization in eukaryotes is thus very complex and tightly controlled. Nathalie Sauvonnet’s team deals with intracellular trafficking of eukaryotic cells and how it regulates cell and tissue organization. In particular we study endocytosis and exocytosis pathways and their linked to lipid distribution, cell compartmentalization and host-pathogen interactions. For this purpose we used an array of techniques from high resolution microscopy, single molecules tracking, robust image analysis, statistical classification, crispR-cas9 edition to organ-on-a-chip.