Guillaume Pidoux

My group focuses on the spatiotemporal regulation of cAMP signaling in cardiac physiological and pathophysiological conditions. The heart ensures that peripheral organs are perfused with blood and nutrients. Any alterations of this function lead to heart injury and failure. A-Kinase Anchoring Proteins (AKAPs) are a family of scaffolding proteins that anchors Protein Kinase-A (PKA), and thus plays a central role in the regulation of cardiac function under physiological and pathological conditions. We focus on understanding the direct role of AKAPs in cardiac physiology and during the occurrence of cardiomyopathies. We utilize molecular biology tools to direct shRNA mediated knockdown and reconstitution assays combined with biochemical assays (i.e. Immunoblot, co-immunoprecipitation, TR-FRET and Alpha-Screen). We further support our studies with state-of-the-art confocal imaging (i.e. live-cell FRAP & FLIP, Proximity Ligation Assay and Super-Resolution imaging). Furthermore, we aim to develop Protein-Protein Interactor (PPI) disrupting peptides to counteract anchored-PKA dependent cellular processes that are modified during cardiac pathologies. These specific PPIs could serve as new potential specific cardiac therapeutic agents. http://cvscience.aviesan.fr/cv/1406/guillaume-pidoux