The team of I. Maridonneau-Parini at the Institute of Pharmacology and Structural Biology (IPBS) is looking for a postdoctoral candidate (for Sidaction application) in charge of deciphering the molecular mechanisms involved in cell-to-cell transfer of HIV-1 to human macrophages and osteoclasts. In addition to T lymphocytes, macrophages and osteoclasts are cell targets for HIV-1 that play crucial roles in the physiopathology of infection including virus dissemination and formation of viral reservoirs (1-5). While HIV-1 infection of macrophages with cell-free viruses has been documented, the mechanisms that control virus dissemination by cell-to-cell transfer are not understood. Interestingly, cell-mediated infection is highly efficient and enables virus escape from antiretroviral drugs and the immune system.
Our team is well-recognized in the field of HIV-1 infection and in the cell biology of macrophages and osteoclasts with an unique expertise on podosomes (6-9), the main F-actin structure in both cell types (6-10). We have reported that HIV-1 modulates essential biological functions of macrophages and osteoclasts, including podosome reorganization (1-2, 4-5). Since podosomes and virological synapses share common components, our objective is to precisely identify the F-actin structures involved in cell-to-cell transfer of the virus, in both human monocyte-derived macrophages and osteoclasts.
In this context, the candidate will be part of an interdisciplinary research program that combines cutting-edge and innovative techniques in biophysics and cell imaging. We will (i) determine the nanoscale architecture of cell-to-cell contacts (3D nanoscopy (6) and lipid bi-layer system to model the surface of HIV-1-infected cells), and (ii) study the kinetics of recruitment and the role of F-actin-associated proteins (microfluidic-based cell pairing system), for efficient virus transfer to macrophages or osteoclasts.
Team Migration and Differentiation of Phagocytes
IPBS, CNRS UMR 5089, Toulouse, France
1-Verollet, C. et al. Blood 125, 1611-1622. 2015
2-Verollet, C. et al. J Immunol 184, 7030-7039. 2010
3-Dupont, M. et al. Front Immunol 9, 43. 2018
4-Raynaud-Messina, B. et al. PNAS. 2018
5-Verollet, C.,et al. Front Immunol 6, 514. 2015
6-Bouissou, A. et al. ACS Nano 11, 4028-4040. 2017
7-Cougoule, C.,et al. Blood 115, 1444-1452. 2010
8-Labernadie, A.et al. Nat. Commun. 5, 5343. 2015
9-Labernadie, A.et al. PNAS 107. 2010
10-Verollet, C. et al. FASEB J 27, 3608-3618. 2013
Applicants should be trained in cell biology and have experience in immunology. Experiences in biophysics and in working in a BSL3 would be a plus. The application dead line is April, 16th, for a position to be opened in October 2018 (for 3 years). PhD and/or MD/PhD is required for October 2018.
If you are interested in applying to Sidaction for a post-doctoral position on this project, please send a motivation letter and a CV to firstname.lastname@example.org.
L. Malaquin, LAAS CNRS, Toulouse, France
S. Benichou, Institut Cochin, Paris, FranceDétails du téléchargement